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Contributor Information

  • Name Ron Laskey
  • Institute University of Cambridge

Tool Details

  • Tool name: Anti-MCM5 [A2.12A7.C10.D9.D10]
  • Alternate names: Minichromosome Maintenance Complex Component 5; P1-CDC46; CDC46; Minichromosome Maintenance Deficient 5 (Cell Division Cycle 46); EC 3.6.4.12
  • Clone: A2.12A7.C10.D9.D10
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IF ; WB
  • Strain: Balb/c
  • Description: MCM5 (Mini Chromosome Maintainance protein-5) is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. MCM5 is a member of the MCM family of chromatin-binding proteins, which have DNA dependent ATPase motifs in their central domain. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may have a role in cell cycle regulation. MCM proteins 2-7 form a family of DNA helicases implicated at the initiation step of DNA synthesis. It has been reported that immunocytochemical assessment of MCM5 expression may be used as a diagnostic tool for cervical cancer and urothelial neoplasia. MCM5 expression may also be applicable in the detection of a number of other cancers including bladder, lung and oesophageal.
  • Immunogen: Bacterially expressed His-tagged human Mcm5 (amino acids 367-582)
  • Isotype: IgG2b
  • Research area: Epigenetics & Nuclear Signalling
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Mini Chromosome Maintenance protein5 (MCM5)
  • Target background: MCM5 (Mini Chromosome Maintainance protein-5) is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. MCM5 is a member of the MCM family of chromatin-binding proteins, which have DNA dependent ATPase motifs in their central domain. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may have a role in cell cycle regulation. MCM proteins 2-7 form a family of DNA helicases implicated at the initiation step of DNA synthesis. It has been reported that immunocytochemical assessment of MCM5 expression may be used as a diagnostic tool for cervical cancer and urothelial neoplasia. MCM5 expression may also be applicable in the detection of a number of other cancers including bladder, lung and oesophageal.

Application Details

  • Application: IF ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Stoeber et al. 2002. J Natl Cancer Inst. 94(14):1071-9. PMID: 12122098.
  •   Diagnosis of genito-urinary tract cancer by detection of minichromosome maintenance 5 protein in urine sediments.