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Contributor Information

  • Name Chris Dean
  • Institute The Institute of Cancer Research

Tool Details

  • Tool name: Anti-ErbB2 [ICR52]
  • Alternate names: ERBB2; Tyrosine kinase type cell surface receptor HER2
  • Clone: ICR52
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Rat
  • Molecular weight of the target: 77 kDa
  • Application: IHC
  • Description: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • Immunogen: Paper not available
  • Isotype: IgG2b
  • Research area: Cancer ; Cell Type or Organelle Marker ; Cell Signaling & Signal Transduction

  • For Research Use Only

Target Details

  • Target: Erb-B2
  • Target molecular weight: 77 kDa
  • Target background: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Application Details

  • Application: IHC

Handling

  • Format: Liquid
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Styles et al. 1990. Int J Cancer. 45(2):320-4. PMID: 1689275.
  •   Rat monoclonal antibodies to the external domain of the product of the C-erbB-2 proto-oncogene.