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Contributor Information

  • Name Arnoud Sonnenberg
  • Institute Netherlands Cancer Institute

Tool Details

  • Tool name: Anti-Integrin a-6B [6B4]
  • Alternate names: ITGA6; CD49F Antigen
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; WB
  • Strain: Balb/c
  • Description: The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Specific loss of this integrin chain in the intestinal epithelium, and thus of their hemidesmosomes, induces long-standing colitis and infiltrating adenocarcinomas.
  • Immunogen: A mouse was immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin a6B including an appending N-terminal cysteine: CSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKK coupled to keyhole limpet hemocyanin.
  • Isotype: IgG1
  • Research area: Adhesion ; Cell Signaling & Signal Transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Integrin a6B
  • Target background: The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Specific loss of this integrin chain in the intestinal epithelium, and thus of their hemidesmosomes, induces long-standing colitis and infiltrating adenocarcinomas.

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Hogervorst et al. 1993. J Cell Biol. 121(1):179-91. PMID: 7681434.