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Contributor Information

  • Institute Yale University

Tool Details

  • Tool name: Anti-IAb +Ea peptide [YAe]
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Description: Monoclonal antibody which binds YAe antigen on B cells and antigen presenting cells. Background and Research Application This YAe antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The YAe antibody binds to an epitope expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This epitope is found on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the YAe antigen is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. This antibody does not react with invariant chain-associated class II MHC complexes.
  • Immunogen: P14483
  • Immunogen UniProt ID: P14483
  • Research area: Immunology

  • For Research Use Only

Target Details

  • Target: MHC Class II (IA/IE)
  • Target background: Monoclonal antibody which binds YAe antigen on B cells and antigen presenting cells. Background and Research Application This YAe antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The YAe antibody binds to an epitope expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This epitope is found on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the YAe antigen is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. This antibody does not react with invariant chain-associated class II MHC complexes.

Application Details

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Bonacina et al. 2018. Nat Commun. 9(1):3083. PMID: 30082772.
  •   Rouhani et al. 2015. Nat Commun. 6:6771. PMID: 25857745.
  •   Michelet et al. 2015. J Immunol. 194(5):2079-88. PMID: 25637027.
  •   Murphy et al. 1989. Nature. 338(6218):765-8. PMID: 2469959.