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Contributor Information

  • Name Tony Marion
  • Institute The University of Tennessee Health Science Center (UTHSC)

Tool Details

  • Tool name: Anti-DNA [m17-s13]
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Application: ELISA
  • Description: Monoclonal anti-DNA antibodies were generated from a spontaneous mouse model of Systemic Lupus Erythematosus (SLE) (NZB x NZW)F1 using standard methodologies for the generation of B-cell hybridomas. The mice spontaneously developed anti-DNA antibodies that contributed to SLE disease. The mice were neither immunized nor stimulated non-specifically. Hybridomas derived from these autoimmune mice provide the opportunity to analyse the structure, function, and biology of autoantibodies important to understanding their contribution to the pathogenesis of SLE. Table 1 provides a summary of the variable region structures and DNA specificity for the monoclonal anti-DNA autoantibodies generated.
  • Isotype: IgM
  • Research area: Immunology

  • For Research Use Only

Target Details

  • Target: ssDNA and/or dsDNA
  • Target background: Monoclonal anti-DNA antibodies were generated from a spontaneous mouse model of Systemic Lupus Erythematosus (SLE) (NZB x NZW)F1 using standard methodologies for the generation of B-cell hybridomas. The mice spontaneously developed anti-DNA antibodies that contributed to SLE disease. The mice were neither immunized nor stimulated non-specifically. Hybridomas derived from these autoimmune mice provide the opportunity to analyse the structure, function, and biology of autoantibodies important to understanding their contribution to the pathogenesis of SLE. Table 1 provides a summary of the variable region structures and DNA specificity for the monoclonal anti-DNA autoantibodies generated.

Application Details

  • Application: ELISA

Handling

  • Format: Liquid
  • Storage buffer:
  • Storage conditions:
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Marion et al. 1997. Methods. 11(1):3-11. PMID: 8990083.
  •   Tillman et al. 1992. J Exp Med. 176(3):761-79. PMID: 1512540.
  •   Marion et al. 1982. J Immunol. 128(2):668-74. PMID: 7198664.