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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-CyclinA [E72.1]
  • Alternate names: CCNA1; Cyclin A1; Testicular Tissue Protein Li 34; CT146
  • Clone: E72.1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Bovine
  • Host: Mouse
  • Application: IHC ; IF ; IP ; WB
  • Description: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
  • Immunogen: Bovine Cyclin A
  • Isotype: IgG1
  • Research area: Cancer ; Cell Cycle ; Epigenetics & Nuclear Signalling
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Cyclin A
  • Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Garner et al. 2002. Oncogene. 21(53):8089-104. PMID: 12444545.
  •   Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
  •   Bashir et al. 2000. Proc Natl Acad Sci U S A. 97(10):5522-7. PMID: 10792046.
  •   Cyclin A activates the DNA polymerase delta -dependent elongation machinery in vitro: A parvovirus DNA replication model.