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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-CAT [CAT-1]
  • Clone: CAT-1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Molecular weight of the target: 26000 kDa
  • Application: WB ; IHC ; IF ; WB
  • Description: Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene.
  • Immunogen: Full length Chloramphenicol acetyl transferase
  • Isotype: IgG1
  • Research area: Cell Type or Organelle Marker; Gene Expression
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Chloramphenicol acetyl transferase (CAT)
  • Target molecular weight: 26000 kDa
  • Target background: Bacterial chloramphenicol acetyl transferase (CAT) is an enzyme that catalyzes the inactivation of the antibiotic, chloramphenicol, by acetylation and subsequently confers bacterial resistance to the antibiotic. CAT, being a stable prokaryotic enzyme, is often used as a reporter gene in transfection assays developed for eukaryotic promoters. Quantification of reporter gene expressions, such as that of CAT, can be correlated to the transcriptional functions of the target sequence. Thus, antibodies directed against CAT can be used for the study of gene sequences that are fused to the CAT reporter gene.

Application Details

  • Application: WB ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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  • Available on request

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References

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  • •  Persson et al. 2017. Am J Physiol Renal Physiol. 312(2):F278-F283. PMID: 27927650.
  • •  Mitchell et al. 2017. J Int Soc Sports Nutr. 14:17. PMID: 28603468.
  • •  Sheen et al. 2016. Front Cell Neurosci. 10:167. PMID: 27445694.
  • •  Kishikawa et al. 2015. Oncotarget. 6(10):8339-52. PMID: 25826076.
  • •  Wang et al. 2014. Biol Reprod. 91(3):59. PMID: 25061098.
  • •  Wang et al. 2014. Biol Reprod. 90(4):84. PMID: 24648395.
  • •  Battisti et al. 2012. Am J Respir Cell Mol Biol. 46(4):498-506. PMID: 22074703.
  • •  Barilli et al. 2011. J Leukoc Biol. 90(2):293-303. PMID: 21586674.
  • •  Kuang et al. 2007. Am J Physiol Lung Cell Mol Physiol. 292(4):L944-52. PMID: 17209135.
  • •  Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts.