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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CR1 [E11]
  • Clone: E11
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IP
  • Strain: Balb/c
  • Description: Monoclonal antibody which binds to a specific epitope in complement receptor 1 without effecting complement activity, with use in CR1 quantitation.
  • Immunogen: Acute monocytic leukaemia cells and normal blood monocytes.
  • Immunogen UniProt ID: P17927
  • Isotype: IgG1
  • Research area: Cancer ; Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Complement receptor 1 (CR1, CD35)
  • Target background: CR1 is found on many leucocytes and binds C3b and C4b. E11 detects CR1 on most leukemic monoblasts, granulocytes and may be used to define B cell lymphomas. E11 binds to a 222kDa antigen. This antibody is specific for a binding site in CR1 which is distal from the C3b binding site. As such CR1 activity is not blocked. CR1 is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b within the complement system. CR1 is present on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukaemia's, follicular dendritic reticulum cells and is negative on basophils. Anti-CR1 E11 is useful for identifying CR1 on various cells in tissue sections, and for quantitating CR1 on erythrocytes and neutrophils.

Application Details

  • Application: IHC ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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  • Available on request

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References

  • •  Leucocyte Typing III. Edited by AJ McMichael et al. 1987
  • •  Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820.
  • •  Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463.
  • •  Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996.
  • •  Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
  • •  Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
  • •  Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248.
  • •  Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).