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Contributor Information

  • Name Birgit Lane
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Keratin8 [LP3K]
  • Clone: LP3K
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 52 kDa
  • Application: IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.
  • Immunogen: Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes.
  • Isotype: IgG1
  • Research area: Cell Signaling & Signal Transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Keratin 8
  • Target molecular weight: 52 kDa
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.

Application Details

  • Application: IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.
  •   Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.
  •   Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.
  •   The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.
  •   Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.
  •   Keratin antigens in differentiating skin.