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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-ARF1 [ARFS 3F1]
  • Clone: ARFS 3F1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.
  • Immunogen: C-terminal Human ARF1 peptide SNQLRNQ
  • Isotype: IgG2a
  • Research area: Cell Signaling & Signal Transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: ADP-Ribosylation Factor 1 (ARF1)
  • Target background: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.

Application Details

  • Application: ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Lim et al. 2010. Genes Dev. 24(14):1496-506. PMID: 20634316.
  •   The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling.
  •   Yano et al. 2008. Mol Biol Cell. 19(3):822-32. PMID: 18094045.
  •   Fbx8 makes Arf6 refractory to function via ubiquitination.
  •   Cotton et al. 2007. Mol Biol Cell. 18(2):501-11. PMID: 17122362.
  •   Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration.
  •   Austin et al. 2000. J Biol Chem. 275(29):21862-9. PMID: 10807927.
  •   Direct and GTP-dependent interaction of ADP-ribosylation factor 1 with clathrin adaptor protein AP-1 on immature secretory granules.