ANTIBODIES
Contributor Information
- Name Sharon Tooze
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-ARF1 [ARFS 3F1]
- Clone: ARFS 3F1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; IF ; WB
- Strain: Balb/c
- Description: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.
- Immunogen: C-terminal Human ARF1 peptide SNQLRNQ
- Isotype: IgG2a
- Research area: Cell Signaling & Signal Transduction
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: ADP-Ribosylation Factor 1 (ARF1)
- Target background: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.
Application Details
- Application: ELISA ; IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
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References
- • Lim et al. 2010. Genes Dev. 24(14):1496-506. PMID: 20634316.
- • The CNK1 scaffold binds cytohesins and promotes insulin pathway signaling.
- • Yano et al. 2008. Mol Biol Cell. 19(3):822-32. PMID: 18094045.
- • Fbx8 makes Arf6 refractory to function via ubiquitination.
- • Cotton et al. 2007. Mol Biol Cell. 18(2):501-11. PMID: 17122362.
- • Endogenous ARF6 interacts with Rac1 upon angiotensin II stimulation to regulate membrane ruffling and cell migration.
- • Austin et al. 2000. J Biol Chem. 275(29):21862-9. PMID: 10807927.
- • Direct and GTP-dependent interaction of ADP-ribosylation factor 1 with clathrin adaptor protein AP-1 on immature secretory granules.