Product Image

Contributor Information

  • Name Stephen West
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Rad51B [Rad51B 1E11/6]
  • Clone: Rad51B 1E11/6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Hamster
  • Host: Mouse
  • Molecular weight of the target:
  • Application: WB
  • Strain: Balb/c
  • Description: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
  • Immunogen: Raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification using SDS-PAGE.
  • Isotype: IgG1
  • Research area: DNA Damage and Repair ; Epigenetics & Nuclear Signalling
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Rad51B
  • Target molecular weight:
  • Target background: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
  •   Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.