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Contributor Information

  • Name Stephen West
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Rad51C [Rad51C 2H11/6]
  • Clone: Rad51C 2H11/6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Molecular weight of the target:
  • Application: IF ; IHC ; IP ; WB
  • Strain: Balb/c
  • Description: RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway.
  • Immunogen: Rad51C 2H11/6 was raised against His-tagged human Rad51C, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page
  • Isotype: IgG1
  • Research area: DNA Damage and Repair ; Epigenetics & Nuclear Signalling
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Rad51C
  • Target molecular weight:
  • Target background: RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway.

Application Details

  • Application: IF ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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  • Available on request

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References

  • •  Park et al. 2014. Oncogene. 33(40):4803-12. PMID: 24141787.
  • •  Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
  • •  French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
  • •  Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
  • •  Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
  • •  Identification and purification of two distinct complexes containing the five RAD51 paralogs.
  • •  Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
  • •  Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
  • •  Dosanjh et al. 1998. Nucleic Acids Res. 26(5):1179-84. PMID: 9469824.
  • •  Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.