ANTIBODIES

Contributor Information
- Name Helen Turley
- Institute University of Oxford
Tool Details
- Tool name: Anti-PHD1 [PHD112/G7]
- Clone: PHD112/G7
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 43.6 kDa
- Application: IHC ; WB
- Strain: Balb/c
- Description: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.
- Immunogen: Full length recombinant human PHD1
- Isotype: IgM
- Research area: Cancer; Epigenetics & Nuclear Signalling; Metabolism
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: Prolyl Hydroxylase 1 (PHD1)
- Target molecular weight: 43.6 kDa
- Target background: PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.
Application Details
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
Related Tools
References
- • Andersen et al. 2011. PLoS One. 6(8):e23847. PMID: 21887331.
- • Overexpression of the HIF hydroxylases PHD1, PHD2, PHD3 and FIH are individually and collectively unfavorable prognosticators for NSCLC survival.
- • Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.
- • Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.
- • Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.
- • Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.
- • Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].
- • Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.