Product Image

Contributor Information

  • Name Karen Pulford
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-Leu2 [X107]
  • Alternate names: CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen; Alpha Polypeptide (P32); MAL; T-Cell Surface Glycoprotein CD8 Alpha Chain; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T8 T-Cell Antigen; CD8a Antigen; Leu2; P32
  • Clone: X107
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC
  • Description: Leu-2 (CD8) is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. Leu-2 is present on human suppressor / cytotoxic T cells which make up 30% of circulating T cells. X107 may be used to detect these cells in tissue sections and also their neoplastic counterparts.
  • Immunogen: Synthetic peptide ( the 13 c-terminal residues of the cytoplasmic domain of human CD8a)
  • Isotype: Not Known
  • Research area: Immunology ; Stem Cell Biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Leu2 (CD8)
  • Target background: Leu-2 (CD8) is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. Leu-2 is present on human suppressor / cytotoxic T cells which make up 30% of circulating T cells. X107 may be used to detect these cells in tissue sections and also their neoplastic counterparts.

Application Details

  • Application: FACS ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Knapp W. et al. 1989. Leukocyte Typing IV. Oxford University Press. p 1076. ISBN-13: 978-0192618672