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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Ufd1 [5e2]
  • Clone: 500
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Rat ; Mouse ; Xenopus laevis
  • Host: Mouse
  • Molecular weight of the target: 40 kDa
  • Application: ELISA ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
  • Immunogen: Full length mouse Ufd1 expressed in E. coli
  • Isotype: IgG2a
  • Research area: Cancer ; Cell Cycle
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Ufd1
  • Target molecular weight: 40 kDa
  • Target background: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.

Application Details

  • Application: ELISA ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
  •   The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
  •   Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
  •   Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
  •   Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
  •   A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.