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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-FBW7 [FBOX 3a9/1]
  • Alternate names: F-Box And WD Repeat Domain Containing 7; F-Box Protein FBX3; SEL-1; Fbx3; HCdc4; FBW7; Hago; Homolog Of C Elegans Sel-1; F-Box Protein SEL-1; Archipelago Homolog; F-Box Protein FBW7; Archipelago; FBXO3; FBXW6; CDC4; FBW6; AGO
  • Clone: FBOX 3a9/1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: SCF (Skp, Cullin, F-box containing complex) ubiquitin ligases regulate the degradation of many proteins involved in the control of cell division and growth. F-box proteins are the SCF components that bind to substrates, and this binding is usually signaled by substrate phosphorylation. The FBW7/Cdc4 F-box protein was first recognized by its ability to bind cyclin E, and the SCF (FBW7) is now known to target c-Myc, c-Jun and Notch for degradation in addition to its role in cyclin E proteolysis. FBW7 thus negatively regulates several key oncoproteins. Accordingly, FBW7 is a tumor suppressor that is mutated in a wide spectrum of human cancers, and FBW7 functions as a haplo-insufficient tumor suppressor in mice.
  • Immunogen: Synthetic peptide of human sequence
  • Isotype: IgG1
  • Research area: Cancer ; Protein Degradation
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: F-box/WD repeat-containing protein 7 (FBW7, Cdc4)
  • Target background: SCF (Skp, Cullin, F-box containing complex) ubiquitin ligases regulate the degradation of many proteins involved in the control of cell division and growth. F-box proteins are the SCF components that bind to substrates, and this binding is usually signaled by substrate phosphorylation. The FBW7/Cdc4 F-box protein was first recognized by its ability to bind cyclin E, and the SCF (FBW7) is now known to target c-Myc, c-Jun and Notch for degradation in addition to its role in cyclin E proteolysis. FBW7 thus negatively regulates several key oncoproteins. Accordingly, FBW7 is a tumor suppressor that is mutated in a wide spectrum of human cancers, and FBW7 functions as a haplo-insufficient tumor suppressor in mice.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Tu et al. 2013. PLoS One. 8(7):e68574. PMID: 23840897.
  •   Recombinant human adenovirus-p53 injection induced apoptosis in hepatocellular carcinoma cell lines mediated by p53-Fbxw7 pathway, which controls c-Myc and cyclin E.
  •   Nateri et al. 2004. Science. 303(5662):1374-8. PMID: 14739463.
  •   The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling.