ANTIBODIES
Contributor Information
- Name Walter Bodmer
- Institute University of Oxford
Tool Details
- Tool name: Anti-LewisA [PR 5C5] mAb
- Clone: PR 5C5
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC
- Strain: Balb/c
- Description: PR 5C5 is useful for histological diagnosis of colon disorders and has wide reactivity with bowel, small intestine, stomach and colo-rectal tumours.
- Immunogen: Mucosal scrapings and membrane preparations from normal colorectal epithelium and possibly booster inoculations with the colon carcinoma cell line HT29.
- Isotype: IgG1
- Research area: Tissue-specific biology; Immunology
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: Lewis A antigen
- Target background: Lewis a antigen is expressed on colonic mucus, epithelial cells and cell membranes. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Application Details
- Application: ELISA ; IHC
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Documentation
Related Tools
References
- • Richman et al. 1987. Int J Cancer. 39(3):317-28. PMID: 2434440.
- • Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization.
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