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Contributor Information

  • Name Walter Bodmer
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-LewisA [PR 5C5] mAb
  • Clone: PR 5C5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC
  • Strain: Balb/c
  • Description: PR 5C5 is useful for histological diagnosis of colon disorders and has wide reactivity with bowel, small intestine, stomach and colo-rectal tumours.
  • Immunogen: Mucosal scrapings and membrane preparations from normal colorectal epithelium and possibly booster inoculations with the colon carcinoma cell line HT29.
  • Isotype: IgG1
  • Research area: Tissue-specific biology; Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Lewis A antigen
  • Target background: Lewis a antigen is expressed on colonic mucus, epithelial cells and cell membranes. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Application Details

  • Application: ELISA ; IHC


  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C



  •   Richman et al. 1987. Int J Cancer. 39(3):317-28. PMID: 2434440.
  •   Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization.