ANTIBODIES
Contributor Information
- Name Paul Crocker
- Institute University of Dundee
Tool Details
- Tool name: Anti-SIGLEC8 [7C9]
- Clone: 7C9
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IP
- Strain: Balb/c
- Description: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
- Immunogen: Siglec-8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of human IgG1
- Isotype: IgG1 kappa
- Research area: Adhesion; Cell Type or Organelle Marker; Cell Signaling & Signal Transduction; Immunology
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: SIGLEC8
- Target background: SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
Application Details
- Application: ELISA ; FACS ; IP
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: DMEM + 10% Fetal Clone I + 1% penecillin/streptomycin
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
Related Tools
References
- • Floyd et al. 2000. J Biol Chem. 275:861-6 PMID: 10625619 Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.
.svg)
Unit size: 
.svg)
.svg)
