ANTIBODIES

Contributor Information
- Name Geoff Toms
- Institute Newcastle University
Tool Details
- Tool name: Anti-RSV F Glycoprotein [5A6]
- Clone: 5A6
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Respiratory Syncytial Virus
- Host: Mouse
- Application: WB ; ELISA ; IHC; IF ; WB
- Description: RSV5A6 may be used for the detection of the fusion glycoprotein of HRSV of both sub-groups A and B.
- Immunogen: hRSV strain A2 infected HeLa cells
- Isotype: IgG2a
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: Respiratory syncytial virus subgroup A G glycoprotein. Epitope is less defined. As well as ELISA the antibody binds the F (specifically the F1) polypeptide in Western blots. It also binds to the F polypeptide expressed in E. coli but not to any of a set of overlapping fragment of F similarly expressed.
- Target background: RSV5A6 may be used for the detection of the fusion glycoprotein of HRSV of both sub-groups A and B.
Application Details
- Application: WB ; ELISA ; IHC; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: RPMI + 10% FCS + Hepes (25mM) + glutamine (2mM)
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
References
- • West et al. 1994. J Gen Virol. 75:2813-9. PMID: 7931172 Wright et al. 1997. J Pathol. 182:238-44 PMID: 9274537