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Contributor Information

  • Institute A*STAR Accelerate Technologies Pte Ltd

Tool Details

  • Tool name: Anti-SDelta3 [7G12]
  • Clone: 7G12
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Virus
  • Host: Mouse
  • Application: IP ; WB
  • Description: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The S?3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.
  • Immunogen: GST-S?3 fusion protein
  • Isotype: IgG1
  • Research area: Microbiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: S protein of the SARS coronavirus
  • Target background: Monoclonal antibody used to aid understanding the method of SARS membrane fusion and entry. Background and Research Application Severe acute respiratory syndrome coronavirus (SARS-CoV) is a species of coronavirus known to infect humans, bats, and certain other mammals. The SARS-related coronavirus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by the ACE2 receptor. SARS genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. The SΔ3 is a fragment containing residues from 168 to 461 of the S protein. This antibody has use in investigating the mechanism of membrane fusion and virus entry.

Application Details

  • Application: IP ; WB
  • Application notes: Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody was found to have no viral neutralizing activity (Lip et al., 2006). Concentration 1mg/ml as standard

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
  • •  Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
  • •  Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.