ANTIBODIES
Contributor Information
- Name Lindy Durrant
- Institute University of Nottingham
Tool Details
- Tool name: Anti-Lewis y/b hapten [NCRC37]
- Clone: NCRC37
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; Fn
- Strain: Balb/c
- Description: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
- Immunogen: Tissue cultured colorectal tumor cell lines (C170, C146, Colo205, and JW)
- Isotype: IgG1
- Research area: Immunology
- Myeloma used: NS0
- For Research Use Only
Target Details
- Target: Sialyltetraosylceramiden antigen on colorectal tumor cell surface
- Target background: Blood group related antigens are a group of carbohydrate determinants present on both glycoproteins and glycolipids. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Lewis- y/b is a cell surface carbohydrate blood group antigen expressed at very low levels in normal tissue, but often found to be over-expressed on breast, lung, colon and ovarian cancer cells. Lewis-y antigen, a difucosylatedtetrasaccharide, is found on type 2 blood group oligosaccharides. The antibody, NCRC37, is a pan-reactive, anti-colorectal tumour antibody and binds strongly to coloretal tumour cells and to post mortem cryopreserved tissues of brain, spleen, heat, lung, kidney, testes, oesophagus or stomach. Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Application Details
- Application: ELISA ; FACS ; IHC ; Fn
Handling
- Format: Liquid
- Concentration: 1.14 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
References
- • Durrant et al. 2006. Cancer Res. 66(11):5901-9. PMID: 16740730.
- • A new anticancer glycolipid monoclonal antibody, SC104, which directly induces tumor cell apoptosis.
- • Durrant et al. 1993. Hybridoma. 12(6):647-60. PMID: 7507082.
- • Development of second generation monoclonal antibodies recognising Lewisy/b antigen by anti-idiotypic immunisation.
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