ANTIBODIES
Contributor Information
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti-Nesprin-2 [6C11]
- Alternate names: Nesp2 antibody, Nesprin-2 antibody, Nuclear envelope spectrin repeat protein 2 antibody
- Clone: 6C11
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IF ; WB
- Strain: C57BL/6
- Description: A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.
- Immunogen: GST fused to residues 6,653-6,835 of human Nesp2
- Isotype: IgG2b kappa
- Research area: Developmental Biology; Gene Expression
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Nesprin-2
- Target background: A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.
Application Details
- Application: IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
.svg)
Unit size: 
.svg)
.svg)
