ANTIBODIES
Contributor Information
Tool Details
- Tool name: Anti-HuD [15A9] (ChIP Grade)
- Alternate names: ELAV-like protein 4
- Clone: 15A9
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ChIP ; ELISA ; IHC ; IP ; WB
- Description: HuD otherwise known as ELAV-like protein 4 is a protein that in humans is encoded by the ELAVL4 gene. The HuD/ELAVL4 protein is an RNA-binding protein. HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the half-life of the transcript is increased. HuD is important in neurons during brain development and plasticity. Monoclonal 15A9 was generated against a unique HuD peptide and does not react with HuR, HuC, or Hel-N1. Monoclonal 15A9 works well in western blot, IHC and provides an excellent marker for neuronal cells. However, it was specifically selected becasue of its abiity to supershift HuD/mRNA ribonucleoprotein complexes. Thus, it is the ideal reagent for CHIP or RIP studies to specifically identify novel HuD targets in neuronal cells.
- Isotype: IgG2b
- Research area: Developmental Biology; Gene Expression; Neurobiology
- For Research Use Only
Target Details
- Target: HuD
- Target background: HuD otherwise known as ELAV-like protein 4 is a protein that in humans is encoded by the ELAVL4 gene. The HuD/ELAVL4 protein is an RNA-binding protein. HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the half-life of the transcript is increased. HuD is important in neurons during brain development and plasticity. Monoclonal 15A9 was generated against a unique HuD peptide and does not react with HuR, HuC, or Hel-N1. Monoclonal 15A9 works well in western blot, IHC and provides an excellent marker for neuronal cells. However, it was specifically selected becasue of its abiity to supershift HuD/mRNA ribonucleoprotein complexes. Thus, it is the ideal reagent for CHIP or RIP studies to specifically identify novel HuD targets in neuronal cells.
Application Details
- Application: ChIP ; ELISA ; IHC ; IP ; WB
Handling
- Format: Liquid
- Storage buffer:
- Storage conditions:
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
.svg)
Unit size: 
.svg)
.svg)
