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Contributor Information

  • Name Zofia Chrzanowska-Lightowlers
  • Institute Newcastle University

Tool Details

  • Tool name: Anti-mtEF-Tu
  • Alternate names: Mitochondrial Elongation Factor Tu, EF-Tu, P43, Elongation Factor Tu, Mitochondrial, EF-TuMT, COXPD4, EFTU
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: Predicted 43 kDa
  • Application: WB
  • Description: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy
  • Immunogen: Recombinant protein
  • Research area: Genetics

  • For Research Use Only

Target Details

  • Target: Mitochondrial EF-Tu
  • Target molecular weight: Predicted 43 kDa
  • Target background: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: Thaw in RPMI + 10% condimed + 20% FCS + 1x pen and Strep + 1 x L-glutamine (~2.7mM). Once growing serially dilute and reduce condimed from 10% to 5% to 2% to 0%. After that can start to drop FCS from 20% to 15% to 10% or even as low as 5%. When growing sufficiently, then stop feeding, when approx. 80% dead cells look shriveled and medium goes yellowish, then pellet the cells down hard and retain the supernatant. This is then filtered to remove as much non-antibody as possible and used as anti-serum.
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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