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Contributor Information

  • Name Ted Ross
  • Institute University of Georgia

Tool Details

  • Tool name: Anti-Ferret immunoglobulin (IgH) [GK1-2-11-E3]
  • Alternate names: Ig
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Application: WB ; ELISA ; FACS
  • Description: The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79Î?+ B cells. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells, and represent great improvement over polyclonal anti-ferret Ig reagents.
  • Immunogen: Ferret IgG and IgM
  • Isotype: IgG1 kappa
  • Research area: Immunology; Virology

  • For Research Use Only

Target Details

  • Target: Ferret Immunoglobulin
  • Target background: The domestic ferret (Mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. Despite the importance of this animal model, characterization of the immune response by flow cytometry (FCM) is severely hampered due to the limited number of commercially available reagents. To begin to address this unmet need and to facilitate more in-depth study of ferret B cells including the identification of antibody-secreting cells, eight unique murine monoclonal antibodies (mAb) with specificity for ferret immunoglobulin (Ig) were generated using conventional B cell hybridoma technology. These mAb were screened for reactivity against ferret peripheral blood mononuclear cells by FCM and demonstrate specificity for CD79Î?+ B cells. Several of these mAb are specific for the light chain of surface B cell receptor (BCR) and enable segregation of kappa and lambda B cells, and represent great improvement over polyclonal anti-ferret Ig reagents.

Application Details

  • Application: WB ; ELISA ; FACS

Handling

  • Format: Liquid
  • Storage buffer:
  • Storage conditions:
  • Shipping conditions: Shipping at 4°C

Documentation

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  • Available on request

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References

  • •  Kirchenbaum et al. 2017. J Immunol Res. 2017:5874572. PMID: 28286781.
  • •  Kirchenbaum et al. 2017. J Immunol. 199(11):3798-3807. PMID: 29079697.
  • •  Infection of Ferrets with Influenza Virus Elicits a Light Chain-Biased Antibody Response against Hemagglutinin.
  • •  Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo).