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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Anti-Mycobacterial 65-kDa hsp [Rb2]
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mycobacterium bovis ; Mycobacterium avium
  • Host: Mouse
  • Application: ELISA ; IHC ; WB
  • Strain: Balb/c
  • Description: Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44), 85B (MPT59) and 85C (MPT45). This antibody stains clear rod-shaped M.leprae bacilli within macrophages.
  • Immunogen: 65-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114)
  • Isotype: IgG1
  • Research area: Microbiology; Immunology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Mycobacterial 65-kDa hsp
  • Target background: Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44), 85B (MPT59) and 85C (MPT45). This antibody stains clear rod-shaped M.leprae bacilli within macrophages.

Application Details

  • Application: ELISA ; IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: RPMI 1640
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
  •   Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.