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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Anti-Mycobacterial 65-kDa hsp [Pe12]
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mycobacterium bovis ; Mycobacterium avium
  • Host: Mouse
  • Application: ELISA ; IHC ; WB
  • Strain: Balb/c
  • Description: Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44) and 85B (MPT59). This antibody stains clear rod-shaped M.leprae bacilli within macrophages.
  • Immunogen: 65-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114)
  • Isotype: IgG1
  • Research area: Microbiology; Immunology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Mycobacterial 65-kDa hsp
  • Target background: Mycobacterial 65-kDa hsp and the actively secreted antigen 85 complex of 30 -kDa region proteins are considered to be major immune targets in mycobacterial diseases. This antibody recognises hsp65 and antigens 85A (MPT44) and 85B (MPT59). This antibody stains clear rod-shaped M.leprae bacilli within macrophages.

Application Details

  • Application: ELISA ; IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: RPMI 1640
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
  • •  Rambukkana et al. 1992. Infect Immun. 60(11):4517-27. PMID: 1383151.