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Contributor Information

  • Name Pranab K Das

Tool Details

  • Tool name: Anti-Mycobacterial 30-kDa [3A8]
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mycobacterium bovis
  • Host: Mouse
  • Application: ELISA ; IHC ; WB
  • Strain: Balb/c
  • Description: Proteins of the antigen 85 complex in the 30-kDa region secreted by live mycobacteria are important in the immune response against mycobacterial infections and play an important biological role in the host-parasite interaction. This antibody recognises antigens 85A (MPT44), 85B (MPT59) and 85C (MPT45). This antibody stains the paucibacillary borderline and tuberculoid leprosy lesions.
  • Immunogen: 30-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114)
  • Isotype: IgG1
  • Research area: Bacteriology; Immunology; Microbiology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Mycobacterial 30-kDa
  • Target background: Proteins of the antigen 85 complex in the 30-kDa region secreted by live mycobacteria are important in the immune response against mycobacterial infections and play an important biological role in the host-parasite interaction. This antibody recognises antigens 85A (MPT44), 85B (MPT59) and 85C (MPT45). This antibody stains the paucibacillary borderline and tuberculoid leprosy lesions.

Application Details

  • Application: ELISA ; IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1mg/ml
  • Storage buffer: RPMI 1640
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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  • Available on request

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References

  • •  Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
  • •  Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.
  • •  Rambukkana et al. 1992. Scand J Immunol. 36(1):35-48. PMID: 1615282.
  • •  Rambukkana et al. 1991. Scand J Immunol. 33(6):763-75. PMID: 1904624.