ANTIBODIES
Contributor Information
- Institute Netherlands Cancer Institute
Tool Details
- Tool name: Anti-DGKa [M8]
- Alternate names: DGKA; Diacylglycerol Kinase Alpha
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 77 kDa
- Application: IP ; WB
- Strain: Balb/c
- Description: Diacylglycerol kinase alpha is an enzyme that belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signalling pathways. It also plays an important role in the resynthesise of phosphatidylinositol's and phosphorylating diacylglycerol to phosphatidic acid.
- Immunogen: Immunized with an Escherichia coli cell-expressed, affinity-purified glutathione S-transferase protein of a C-terminal portion (part of the Catalytic domain) of Rat DGKu.
- Isotype: IgG1
- Research area: Cell Signaling & Signal Transduction; Immunology; Metabolism
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: DGKa
- Target molecular weight: 77 kDa
- Target background: Diacylglycerol kinase alpha is an enzyme that belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signalling pathways. It also plays an important role in the resynthesise of phosphatidylinositol's and phosphorylating diacylglycerol to phosphatidic acid.
Application Details
- Application: IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15°C to -25°C
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
Related Tools
References
- • Cutrupi et al. 2000. EMBO J. 19(17):4614-22. PMID: 10970854.
- • Schaap et al. 1993. Biochem J. 289 ( Pt 3):875-81. PMID: 7679574.
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