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Contributor Information

  • Name Dawen Cai
  • Institute University of Michigan, USA
  • Primary citation Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127.

Tool Details

  • Tool name: Anti-mNeonGreen (Rabbit) "Brainbow" pAb
  • Alternate names: Anti-mNeonGreen (Rabbit)
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Host: Rabbit
  • Application: IF ; IP ; WB
  • Description: The mNeonGreen polycloncal antibody is one of the Brainbow collection. The antibody host species is a Rabbit. mNeonGreen is a bright monomeric green/yellow fluorescent protein generated from Branchiostoma lanceolatum.

    The brainbow collection has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, drosophila and zebrafish and non-neuronal cells in mice. The Brainbow toolkit allows scientists to image highly complex tissue structures by relying on a stochastic method for making different expression ratio combinations of fluorescent proteins so that structures in proximity can be resolved.
  • Research area: Neurobiology

  • For Research Use Only

Target Details

  • Target: mNeonGreen
  • Target background: mNeonGreen is a fluorescent protein originating from Branchiostoma lanceolatum.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: Serum with 0.02% sodium azide
  • Storage conditions: -80° C
  • Shipping conditions: Shipping at 4° C

Documentation

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References

  • •  Roossien et al. 2019. Bioinformatics. 35(18):3544-3546. PMID: 30715234.
  • •  Chen et al. 2018. Mol Psychiatry. 23(7):1614-1625. PMID: 28761082.
  • •  Takesian et al. 2018. Nat Neurosci. 21(2):218-227. PMID: 29358666.
  • •  Roossien et al. 2017. Methods Mol Biol. 1642:211-228. PMID: 28815503.
  • •  Manent et al. 2017. Oncogene. 36(40):5576-5592. PMID: 28581519.
  • •  Chang et al. 2017. Nat Methods. 14(6):593-599. PMID: 28417997.
  • •  Zanca et al. 2017. Genes Dev. 31(12):1212-1227. PMID: 28724615.
  • •  Tillberg et al. 2016. Nat Biotechnol. 34(9):987-992. PMID: 27376584.
  • •  Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127.