ANTIBODIES
Contributor Information
- Name Tony Marion
- Institute The University of Tennessee Health Science Center (UTHSC)
Tool Details
- Tool name: Anti-DNA [m17-ps-c7]
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Host: Mouse
- Application: ELISA
- Description: Monoclonal anti-DNA antibodies were generated from a spontaneous mouse model of Systemic Lupus Erythematosus (SLE) (NZB x NZW)F1 using standard methodologies for the generation of B-cell hybridomas. The mice spontaneously developed anti-DNA antibodies that contributed to SLE disease. The mice were neither immunized nor stimulated non-specifically. Hybridomas derived from these autoimmune mice provide the opportunity to analyse the structure, function, and biology of autoantibodies important to understanding their contribution to the pathogenesis of SLE. Table 1 provides a summary of the variable region structures and DNA specificity for the monoclonal anti-DNA autoantibodies generated.
- Isotype: IgG2a
- Research area: Immunology
- For Research Use Only
Target Details
- Target: ssDNA and/or dsDNA
- Target background: Monoclonal anti-DNA antibodies were generated from a spontaneous mouse model of Systemic Lupus Erythematosus (SLE) (NZB x NZW)F1 using standard methodologies for the generation of B-cell hybridomas. The mice spontaneously developed anti-DNA antibodies that contributed to SLE disease. The mice were neither immunized nor stimulated non-specifically. Hybridomas derived from these autoimmune mice provide the opportunity to analyse the structure, function, and biology of autoantibodies important to understanding their contribution to the pathogenesis of SLE. Table 1 provides a summary of the variable region structures and DNA specificity for the monoclonal anti-DNA autoantibodies generated.
Application Details
- Application: ELISA
Handling
- Format: Liquid
- Storage buffer:
- Storage conditions:
- Shipping conditions: Shipping at 4°C
Documentation
- Available on request
References
- • Marion et al. 1997. Methods. 11(1):3-11. PMID: 8990083.
- • Tillman et al. 1992. J Exp Med. 176(3):761-79. PMID: 1512540.
- • Marion et al. 1982. J Immunol. 128(2):668-74. PMID: 7198664.
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