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Contributor Information

  • Name Alfonso Colombatti ; Maurizio Mongiat ; Paola Spessotto ; Roberto Doliana ; Maria Teresa Mucignat
  • Institute National Cancer Institute CRO - Aviano

Tool Details

  • Tool name: Anti-hEMILIN1 [hEMIL1]
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; IF ; IP ; Fn ; WB
  • Description: Emilin-1 (Elastin Microfibril Interface Located proteIN) is an extracellular matrix glycoprotein. It is part of a protein family which have been shown to be expressed in smooth muscle and other mesenchymal tissues and is localized at the proximity of elastin and microfibrils. Emilin-1 exerts an important role in lymphatic system, being a crucial structural molecule that regulates the formation of lymphatic capillaries and collectors. Emilin-1 through the interaction with the Îą4β1 integrin via the C-terminal gC1q domain exerts a negative effect on proliferation. It binds pro-TGFβ preventing its maturation to mature TGFβ in the extracellular space, therefore influencing the regulation of blood and lymph vessels formation and maintenance.
  • Immunogen: Recombinant human Emilin-1 as immunizing antigen; Balb/c mice as host
  • Research area: Extracellular Matrix; Cellular Signalling; Integrin Mediated Signalling

  • For Research Use Only

Target Details

  • Target: hEMILIN1
  • Target background: Emilin-1 (Elastin Microfibril Interface Located proteIN) is an extracellular matrix glycoprotein. It is part of a protein family which have been shown to be expressed in smooth muscle and other mesenchymal tissues and is localized at the proximity of elastin and microfibrils. Emilin-1 exerts an important role in lymphatic system, being a crucial structural molecule that regulates the formation of lymphatic capillaries and collectors. Emilin-1 through the interaction with the a4?1 integrin via the C-terminal gC1q domain exerts a negative effect on proliferation. It binds pro-TGF? preventing its maturation to mature TGF? in the extracellular space, therefore influencing the regulation of blood and lymph vessels formation and maintenance.

Application Details

  • Application: ELISA ; IHC ; IF ; IP ; Fn ; WB

Handling

  • Format: Liquid
  • Storage conditions: Store at 2 - 8°C or aliquots at -20°C. Avoid repeated freeze-thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

  • Available on request

References

  •   Capuano et al. 2016. Hum Mutat. 37(1):84-97. PMID: 26462740.
  •   Pivetta et al. 2014. Matrix Biol. 34:22-32. PMID: 24513040.
  •   Danussi et al. 2013. Mol Cell Biol. 33(22):4381-94. PMID: 24019067.
  •   Danussi et al. 2012. Cancer Prev Res (Phila). 5(9):1131-43. PMID: 22827975.
  •   Danussi et al. 2011. J Cell Biol. 195(1):131-45. PMID: 21949412.
  •   Danussi et al. 2008. Mol Cell Biol. 28(12):4026-39. PMID: 18411305.
  •   Spessotto et al. 2006. J Cell Sci. 119(Pt 21):4574-84. PMID: 17074837.
  •   Zacchigna et al. 2006. Cell. 124(5):929-42. PMID: 16530041.
  •   Zanetti et al. 2004. Mol Cell Biol. 24(2):638-50. PMID: 14701737.
  •   Spessotto et al. 2003. J Biol Chem. 278(8):6160-7. PMID: 12456677.
  •   Doliana et al. 1999. J Biol Chem. 274(24):16773-81. PMID: 10358019.