Product Image

Contributor Information

  • Name Bridget Hill
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: TR146 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Buccal mucosa
  • Cancer type: Human neck metastasis
  • Disease: Cancer
  • Conditional: Yes
  • Description: Cell line to investigate buccal mucosa, and as a model for absorption and metabolism of various substances and enzymatically labile drugs. Background and Research Application The TR146 cell line represents a potentially unique in vitro model of buccal mucosa. The cell line has been well characterised and is an ideal tool for permeability and absorption studies. Due to morphological similarities and comparable permeability, excised porcine buccal mucosa has been considered a reasonably good model of human buccal mucosa for drug delivery studies. However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue. TR146 therefore represents a good and potentially unique model of human mucosa. The cells form an undifferentiated, non-keratinised stratified epithelium which shares many characteristics of normal buccal mucosa. TR146 has been characterised for permeability of a number of substances of varying molecular weight and hydrophilicity over a range of pH and osmolality. Activity of aminopeptidase, carboxypeptidase and esterase has also been studied and shown to be comparable with that of human buccal epithelium. The cell line is therefore not only a useful model for absorption but also for metabolism studies with enzymatically labile drugs.
  • Research area: Cancer ; Drug Discovery & Development ; Metabolism

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: 5% CO2; 37°C, HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS), Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Kalu et al. 2017. Oncotarget. 8(49):86369-86383. PMID: 29156801.
  •   Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595.
  •   Pistone et al. 2016. Eur J Pharm Sci. 96:381-389. PMID: 27721043.
  •   Nilsen et al. 2016. Eur J Oral Sci. :. PMID: 27711994.
  •   Methods and terminology used in cell-culture studies of low-dose effects of matrix constituents of polymer resin-based dental materials.
  •   Formulation of polysaccharide-based nanoparticles for local administration into the oral cavity.
  •   Iyire et al. 2016. Sci Rep. 6:32498. PMID: 27581177.
  •   Pre-formulation and systematic evaluation of amino acid assisted permeability of insulin across in vitro buccal cell layers.
  •   Goyer et al. 2016. PLoS One. 11(3):e0149159. PMID: 26933885.
  •   Intestinal Cell Tight Junctions Limit Invasion of Candida albicans through Active Penetration and Endocytosis in the Early Stages of the Interaction of the Fungus with the Intestinal Barrier.
  •   Hyakusoku et al. 2016. J Exp Clin Cancer Res. 35:6. PMID: 26754630.
  •   Zeng et al. 2015. Int J Pharm. 495(2):1028-37. PMID: 26403384.
  •   Poloxamer bioadhesive hydrogel for buccal drug delivery: Cytotoxicity and trans-epithelial permeability evaluations using TR146 human buccal epithelial cell line.
  •   De Ryck et al. 2015. AMB Express. 5:27. PMID: 25995981.
  •   O'Callaghan et al. 2015. Mol Med Rep. :. PMID: 26005189.
  •   Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines.
  •   Microbial inhibition of oral epithelial wound recovery: potential role for quorum sensing molecules?
  •   Chai et al. 2012. J R Soc Interface. 9(77):3528-38. PMID: 22915635.
  •   The biological seal of the implant-soft tissue interface evaluated in a tissue-engineered oral mucosal model.
  •   Mrck Nielsen et al. 2000. Int J Pharm. 200(2):261-70. PMID: 10867256.
  •   TR146 cells grown on filters as a model of human buccal epithelium: V. Enzyme activity of the TR146 cell culture model, human buccal epithelium and porcine buccal epithelium, and permeability of leu-enkephalin.
  •   Nielsen et al. 2000. Int J Pharm. 194(2):155-67. PMID: 10692640.
  •   TR146 cells grown on filters as a model of human buccal epithelium: IV. Permeability of water, mannitol, testosterone and beta-adrenoceptor antagonists. Comparison to human, monkey and porcine buccal mucosa.
  •   Jacobsen et al. 1999. Eur J Pharm Biopharm. 48(3):217-24. PMID: 10612032.
  •   Cyclodextrin inclusion complexes of antimycotics intended to act in the oral cavity--drug supersaturation, toxicity on TR146 cells and release from a delivery system.
  •   Nielsen et al. 1999. Int J Pharm. 185(2):215-25. PMID: 10460917.
  •   Nielsen et al. 1999. J Control Release. 60(2-3):223-33. PMID: 10425328.
  •   TR146 cells grown on filters as a model of human buccal epithelium: permeability of fluorescein isothiocyanate-labelled dextrans in the presence of sodium glycocholate.
  •   TR146 cells grown on filters as a model of human buccal epithelium: III. Permeability enhancement by different pH values, different osmolality values, and bile salts.
  •   Pedersen et al. 1999. Drug Dev Ind Pharm. 25(4):463-70. PMID: 10194601.
  •   Jacobsen et al. 1999. Eur J Oral Sci. 107(2):138-46. PMID: 10232463.
  •   Cyclodextrin inclusion complexes of miconazole and econazole--isolation, toxicity on human cells, and confirmation of a new interpretation of the drug supersaturation phenomenon.
  •   Filter-grown TR146 cells as an in vitro model of human buccal epithelial permeability.
  •   Rupniak et al. 1985. J Natl Cancer Inst. 75(4):621-35. PMID: 2413234.
  •   Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck.