TR146 Cell Line
- Name Bridget Hill
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool name: TR146 Cell Line
Tool type: Cell Lines
Parental cell line:
Tissue: Buccal mucosa
Cancer type: Human neck metastasis
Description: Cell line to investigate buccal mucosa, and as a model for absorption and metabolism of various substances and enzymatically labile drugs.
Background and Research Application
The TR146 cell line represents a potentially unique in vitro model of buccal mucosa. The cell line has been well characterised and is an ideal tool for permeability and absorption studies. Due to morphological similarities and comparable permeability, excised porcine buccal mucosa has been considered a reasonably good model of human buccal mucosa for drug delivery studies. However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue. TR146 therefore represents a good and potentially unique model of human mucosa. The cells form an undifferentiated, non-keratinised stratified epithelium which shares many characteristics of normal buccal mucosa. TR146 has been characterised for permeability of a number of substances of varying molecular weight and hydrophilicity over a range of pH and osmolality. Activity of aminopeptidase, carboxypeptidase and esterase has also been studied and shown to be comparable with that of human buccal epithelium. The cell line is therefore not only a useful model for absorption but also for metabolism studies with enzymatically labile drugs.
Research area: Cancer ; Drug Discovery & Development ; Metabolism
- For Research Use Only
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