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Contributor Information

  • Name Bridget Hill
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
  • Primary citation Rupniak et al. 1985. J Natl Cancer Inst. 75(4):621-35. PMID: 2413234

Tool Details

  • Tool name: TR146 Cell Line
  • Alternate names: Tr146; TR-146; TR 146
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: 67 year old female patient with previous radiotherapy (6,000 rads) and neck dissection
  • Tissue: Buccal mucosa that had infiltrated a lymph node
  • Gender: Female
  • Cancer type: Head and neck cancer; Human neck metastasis; Buccal mucosa squamous cell carcinoma; Squamous cell carcinoma of the oral cavity
  • Growth properties: Adherent
  • Model: Tumourigenic line
  • Model description: TR146 cell ine is tumourigenic in female (nu/nu) mice resulting in the production of rapidly progressing tumours
  • CRISPR: No
  • Application: Human buccal mucosa modeling; Permeability, absorption and metabolism studies of various substances and enzymatically labile drugs; Drug delivery studies
  • Description: Derived from well-differentiated keratinizing squamous cell carcinoma of the buccal mucosa the TR146 cell line represents an in vitro model to study permeability, absorption and metabolism of various enzymatically labile drugs and substances including epithelial-mesenchymal transition (EMT) markers, tight junction proteins and aquaporins.
    Due to morphological similarities and comparable permeability, excised porcine buccal mucosa has been considered a reasonably good model of human buccal mucosa for drug delivery studies. However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue.
    Closely resembling normal human buccal epithelium, the cell line functions as a reliable oral mucosa model.
    TR146 has been characterised for permeability of a number of substances of varying molecular weight and hydrophilicity over a range of pH and osmolality. Activity of aminopeptidase, carboxypeptidase and esterase has also been studied and shown to be comparable with that of human buccal epithelium.
  • Research area: Cancer; Drug development; Metabolism
  • Cellosaurus ID: CVCL_2736
  • Additional notes: Histology: well-differentiated keratinizing squamous cell carcinoma. Cell type: polygonal

  • For Research Use Only

Target Details

Application Details

  • Application: Human buccal mucosa modeling; Permeability, absorption and metabolism studies of various substances and enzymatically labile drugs; Drug delivery studies
  • Application notes: Transport studies involving C-reactive protein; Barrier function assessments using transepithelial electrical resistance and carboxyfluorescein permeability assays; Cytotoxicity studies with antibacterial compounds; Viability and metabolic activity investigations post blue laser light exposure; Candida albicans infection studies; Insulin permeability studies

Handling

  • Format: Frozen
  • Growth medium: HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Volume: 1 ml
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • STR profile: Amelogenin: X; CSF1PO: 11,13; D13S317: 11,14; D16S539: 13; D5S818: 10,11; D7S820: 10,12; THO1: 9; TPOX: 8,9; vWA: 15,18
  • Mycoplasma free: Yes
  • Biosafety level: 2
  • Subculture routine: Split sub-confluent cultures (70-80%) seeding at approximately 1-2 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37C. Cells can reach a maximum density of 7 x 104 cells/cm2

Documentation

References

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