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Contributor Information

  • Name Bridget Hill
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: TR146 Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Buccal mucosa
  • Cancer type: Human neck metastasis
  • Disease: Cancer
  • Growth properties: Undifferentiated, non-keratinsed stratified epithelium, normal human mucosa
  • Model: Tumour line
  • Conditional: Yes
  • Description: Cell line to investigate buccal mucosa, and as a model for absorption and metabolism of various substances and enzymatically labile drugs. Background and Research Application The TR146 cell line represents a potentially unique in vitro model of buccal mucosa. The cell line has been well characterised and is an ideal tool for permeability and absorption studies. Due to morphological similarities and comparable permeability, excised porcine buccal mucosa has been considered a reasonably good model of human buccal mucosa for drug delivery studies. However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue. TR146 therefore represents a good and potentially unique model of human mucosa. The cells form an undifferentiated, non-keratinised stratified epithelium which shares many characteristics of normal buccal mucosa. TR146 has been characterised for permeability of a number of substances of varying molecular weight and hydrophilicity over a range of pH and osmolality. Activity of aminopeptidase, carboxypeptidase and esterase has also been studied and shown to be comparable with that of human buccal epithelium. The cell line is therefore not only a useful model for absorption but also for metabolism studies with enzymatically labile drugs.
  • Research area: Cancer; Drug Discovery & Development; Metabolism
  • Production details: The TR146 cell line originates from a human neck metastasis of a buccal carcinoma, derived from neck node (primary tumour sited in buccal mucosa). Well-differentiated. Female patient, 67 years. Previous radiotherapy (6,000 rads) and neck dissection
  • Cellosaurus ID: CVCL_2736
  • Additional notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 11,13 D13S317: 11,14 D16S539: 13 D5S818: 10,11 D7S820: 10,12 THO1: 9 TPOX: 8,9 vWA: 15,18 Concentration Vial has between 1-5 million cells as standard, however this may vary.

  • For Research Use Only

Target Details

Application Details

  • Application notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 11,13 D13S317: 11,14 D16S539: 13 D5S818: 10,11 D7S820: 10,12 THO1: 9 TPOX: 8,9 vWA: 15,18 Concentration Vial has between 1-5 million cells as standard, however this may vary.

Handling

  • Format: Frozen
  • Growth medium: 5% CO2; 37°C, HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS), Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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  • Available on request

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References

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  • •  TR146 cells grown on filters as a model of human buccal epithelium: V. Enzyme activity of the TR146 cell culture model, human buccal epithelium and porcine buccal epithelium, and permeability of leu-enkephalin.
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  • •  TR146 cells grown on filters as a model of human buccal epithelium: IV. Permeability of water, mannitol, testosterone and beta-adrenoceptor antagonists. Comparison to human, monkey and porcine buccal mucosa.
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  • •  TR146 cells grown on filters as a model of human buccal epithelium: permeability of fluorescein isothiocyanate-labelled dextrans in the presence of sodium glycocholate.
  • •  TR146 cells grown on filters as a model of human buccal epithelium: III. Permeability enhancement by different pH values, different osmolality values, and bile salts.
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  • •  Cyclodextrin inclusion complexes of miconazole and econazole--isolation, toxicity on human cells, and confirmation of a new interpretation of the drug supersaturation phenomenon.
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