CELL LINES
Contributor Information
- Name Joyce Taylor-Papadimitriou
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: 1-7HB2 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MTSV1-7
- Organism: Human
- Tissue: Breast
- Disease: Normal
- Growth properties: Morphology, adhesion properties
- Model: Immortalised Line
- Description: The 1-7HB2 cell line is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. It is a potent tool for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis. MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. The cell line forms branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium and forms spheroids in collagen type I gels.
- Research area: Cell biology
- Cellosaurus ID: CVCL_2261
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Hydrocortisone (5 ??g/ml) + Bovine Insulin (10 ??g/ml).
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.
- • Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.
.svg)
Unit size: 
.svg)
.svg)
