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Contributor Information

  • Name Joyce Taylor-Papadimitriou
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: 1-7HB2 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MTSV1-7
  • Organism: Human
  • Tissue: Breast
  • Description: The 1-7HB2 cell line is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. It is a potent tool for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis. MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. The cell line forms branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium and forms spheroids in collagen type I gels.
  • Research area: Cell Structure and Motility

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Hydrocortisone (5 Âľg/ml) + Bovine Insulin (10 Âľg/ml).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.
  •   Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.