CELL LINES
Contributor Information
- Name Mary Collins
- Institute The Institute of Cancer Research
Tool Details
- Tool name: FLYRD18 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HT 1080
- Organism: Human
- Cancer type: Sarcoma
- Disease: Cancer
- Growth properties: Recombinant retroviral production
- Model: Packaging
- Conditional: Yes
- Description: The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and cat endogenous virus RD114 virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer.
- Research area: Genetics
- Production details: Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248
- Cellosaurus ID: CVCL_8871
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18.
- • Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
- • High-titer packaging cells producing recombinant retroviruses resistant to human serum.
- • Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
- • Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
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