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Contributor Information

  • Name M.S. Hamilton
  • Institute University of Birmingham

Tool Details

  • Tool name: JIM1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Primary
  • Organism: Human
  • Tissue: Blood
  • Gender: Female
  • Cancer type: Human multiple myeloma
  • Disease: Cancer
  • Conditional: Yes
  • Description: JIM1 was established from plasma myeloma cells derived from pleural fluid of an advanced multiple myeloma patient, and is homoplastic with the cell line JIM3. It allows in vitro study and comparison of genetic instability in myeloma tumour lines and in vitro study of the effects of DNA repair deficiency in myeloma tumour lines. JIM1 shows typical plasma cell phenotype (CD38, PCA-1 8A and CD24 positive), deficient in DNA repair.
  • Research area: Cancer ; DNA Damage and Repair ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Dexter culture medium, consisting of Fischer's medium + 20% FCS +10-7 M hydrocortisone sodium succinate. Seed at 2-9 x 10 5 cells/cm3 5% CO2; 37°C
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Brito et al. 2009. Haematologica. 94(1):78-86. PMID: 19059936.
  •   MMSET deregulation affects cell cycle progression and adhesion regulons in t(4
  •   14) myeloma plasma cells.
  •   Leone et al. 2008. Clin Cancer Res. 14(19):6033-41. PMID: 18829482.
  •   Deletions of CDKN2C in multiple myeloma: biological and clinical implications.
  •   Velangi et al. 2004. Carcinogenesis. 25(10):1795-803. PMID: 15142887.
  •   DNA mismatch repair pathway defects in the pathogenesis and evolution of myeloma.
  •   Hamilton et al. 1991. Leukemia. 5(9):768-71. PMID: 1943229.
  •   Normal and neoplastic human plasma cells express bcl-2 antigen.