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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: HEK293A EGFP-LC3 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK293
  • Organism: Human
  • Tissue: Embryo
  • Disease: Cancer, neurodegeneration, infectious diseases
  • Conditional: Yes
  • Description: HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background. This line functions as a reporter cell line to monitor the induction of autophagy after aminoacid starvation or rapamycin treatment as well as autophagosome formation. This cell line uses GFP-LC3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods.
  • Research area: Cancer ; Apoptosis and Programmed Cell Death ; Cell Signaling & Signal Transduction ; Cellular Transport ; Drug Discovery & Development ; Metabolism

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10% FCS + 2mM Glutamine. (400ug/ml geneticin can be added for maintainance but this is not normally necessary as the GFP-LC3 expression level is maintained over at least 20 passages).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.
  •   siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy.
  •   Kchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.
  •   Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes.