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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Joy Burchell
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: 1-7 CE1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MSTV1-7 HER2
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Transgenic
  • Description: The human mammary luminal epithelial cell line MTSV1-7 was transfected with an ERBB2 expression vector, enabling over-expression of the ERBB2 proto-oncogene. It allows in vitro study of ERBB2 proto-oncogene over-expression in human mammary epithelia adn of the effects of ERBB2 over-expression on epithelial morphology & adhesion. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules as E-Cadherin and a2 integrin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
  • Research area: Cell biology; Cancer; Cell signaling and signal transduction; Drug development
  • Production details: MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
  • Cellosaurus ID: CVCL_3033

  • For Research Use Only

Target Details

  • Target: ERBB2

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml +Hycrocortisone at 5ug/ml.
  • Shipping conditions: Dry ice

Documentation

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References

  • •  D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.
  • •  Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.
  • •  D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.
  • •  Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.
  • •  Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.
  • •  Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.