- Name Deborah Barnes
- Institute Cancer Research UK, London Research Insitute: Clare Hall Laboratories
- Tool name: MEF Trex1 KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Disease: Trex1-deficient Aicardi-Goutieres syndrome
- Growth properties: Defective G1/S transition, increased cell doubling time; chronic ATM-dependent checkpoint activation even in the absence of exogenous stress; persistant ssDNA polynucleotide species generated in S phase that accumulates in the cytoplasm.
- Model: Knock-Out
- Conditional: Yes
- Description: The Trex1 KO MEF cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. It allows the study of novel interconnections between DNA replication, DNA damage checkpoint signalling and antiviral-like autoimmune responses. Recapitulates cellular phenotype of Trex1-deficient Aicardi-Goutieres syndrome. Spontaneously transformed clone derived following repeated passaging in culture by standard techniques of primary MEFs from Trex1-/- knockout mouse
- Research area: DNA Damage and Repair; Epigenetics & Nuclear Signalling; Immunology
- Production details: MEF from Trex1-/- knockout mouse
- Cellosaurus ID: CVCL_5A06
- For Research Use Only
- Target: TREX1
- Application notes: Spontaneously transformed clone derived following repeated passaging in culture by standard techniques of primary MEFs from Trex1-/- knockout mouse
- Format: Frozen
- Shipping conditions: Dry ice
- • Yang et al. 2007. Cell. 131(5):873-86. PMID: 18045533.
- • Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.
- • Morita et al. 2004. Mol Cell Biol. 24(15):6719-27. PMID: 15254239.
- • Gene-targeted mice lacking the Trex1 (DNase III) 3'-->5' DNA exonuclease develop inflammatory myocarditis.