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Contributor Information

  • Name Deborah Barnes
  • Institute Cancer Research UK, London Research Insitute: Clare Hall Laboratories

Tool Details

  • Tool name: MEF Trex1 KO Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Trex1-deficient Aicardi-Goutieres syndrome
  • Conditional: Yes
  • Description: The Trex1 KO MEF cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. It allows the study of novel interconnections between DNA replication, DNA damage checkpoint signalling and antiviral-like autoimmune responses. Recapitulates cellular phenotype of Trex1-deficient Aicardi-Goutieres syndrome.
  • Research area: DNA Damage and Repair ; Epigenetics & Nuclear Signalling ; Immunology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Yang et al. 2007. Cell. 131(5):873-86. PMID: 18045533.
  •   Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.
  •   Morita et al. 2004. Mol Cell Biol. 24(15):6719-27. PMID: 15254239.
  •   Gene-targeted mice lacking the Trex1 (DNase III) 3'-->5' DNA exonuclease develop inflammatory myocarditis.