CELL LINES
Contributor Information
- Name Thomas Lindahl
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: MEF UNG KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Cancer type: B cell lymphoma
- Disease: Cancer
- Model: Knock-Out
- Description: The MEF UNG KO Cell Line is a tool for in vivo study of the function of UNG and the effects of DNA mismatch during DNA synthesis. UNG encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway.
- Research area: DNA Damage and Repair
- Production details: Primary MEFs were prepared from embryos, and permanent cell lines established from transformed clones arising spontaneously after repeated passage in culture, by standard procedures
- For Research Use Only
Target Details
- Target: Uracil-DNA Glycosylase (UNG)
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM:F12 (1:1) + 10% FCS.
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
- • Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
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