CELL LINES
Contributor Information
- Name Catrin Pritchard
- Institute University of Leicester
Tool Details
- Tool name: MEF RAF1 KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Model: Knock-Out
- Conditional: Yes
- Description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
- Research area: Cancer; Cell Signaling & Signal Transduction; Drug Discovery & Development; Metabolism
- Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Ă°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
- Cellosaurus ID: CVCL_6D89
- For Research Use Only
Target Details
- Target: RAF1
Application Details
- Application notes: See the Raf-1 knockout mouse in related products.
Handling
- Format: Frozen
- Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • H
ser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227. - • MEK kinase activity is not necessary for Raf-1 function.
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