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Contributor Information

  • Name Catrin Pritchard
  • Institute University of Leicester

Tool Details

  • Tool name: MEF RAF1 KO Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Conditional: Yes
  • Description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Drug Discovery & Development ; Metabolism

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  •   MEK kinase activity is not necessary for Raf-1 function.