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Contributor Information

  • Name Catrin Pritchard
  • Institute University of Leicester

Tool Details

  • Tool name: MEF RAF1 KO Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-Out
  • Conditional: Yes
  • Description: The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
  • Research area: Cancer; Cell signaling and signal transduction; Drug development; Metabolism
  • Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
  • Cellosaurus ID: CVCL_6D89

  • For Research Use Only

Target Details

  • Target: RAF1

Application Details

  • Application notes: See the Raf-1 knockout mouse in related products.


  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  •   MEK kinase activity is not necessary for Raf-1 function.