CELL LINES
Contributor Information
- Name John Marshall
- Institute Queen Mary University of London
Tool Details
- Tool name: A375P beta6-puro Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: A375P
- Organism: Human
- Cancer type: Skin cancer
- Disease: Cancer
- Growth properties: A375P melanoma cells stably expressing integrin beta 6
- Model: Transgenic
- Description: The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
- Research area: Cancer; Cell biology; Cell signaling and signal transduction; Drug development
- Production details: The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerÄË?Â?Âs instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a month. Use the magnetic beads above to re-select when this happens.
- Cellosaurus ID: CVCL_5F66
- For Research Use Only
Target Details
- Target: Integrin beta 6
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM + 10%FCS/1.25ug/ml puromycin. Antibotic is not required all the time.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.
- • Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.
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