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Contributor Information

  • Name John Marshall
  • Institute Queen Mary University of London

Tool Details

  • Tool name: A375P beta6-puro Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: A375P
  • Organism: Human
  • Cancer type: Skin cancer
  • Disease: Cancer
  • Growth properties: A375P melanoma cells stably expressing integrin beta 6
  • Model: Transgenic
  • Description: The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction; Drug development
  • Production details: The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerâ?‚€?‚™s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a month. Use the magnetic beads above to re-select when this happens.
  • Cellosaurus ID: CVCL_5F66

  • For Research Use Only

Target Details

  • Target: Integrin beta 6

Application Details


  • Format: Frozen
  • Growth medium: DMEM + 10%FCS/1.25ug/ml puromycin. Antibotic is not required all the time.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.
  •   Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.