Product Image

Contributor Information

  • Name Catrin Pritchard
  • Institute University of Leicester

Tool Details

  • Tool name: MEF Raf1 FF/FF KI Cell Line
  • Tool type: Cell Lines
  • Parental cell line: See the Raf KI FF knockout mouse that these cells were derived from in related products.
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Model: Knock-In
  • Conditional: Yes
  • Description: The MEF Raf1 FF/FF KI Cell Line is a MEF line obtained from the knock-in mouse carrying inactive Raf-1FF/FF mutant.
  • Research area: Cancer; Cell signaling and signal transduction; Drug development; Metabolism
  • Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4Â?‚°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
  • Cellosaurus ID: CVCL_6D90

  • For Research Use Only

Target Details

  • Target: Raf1 Y340F Y341F inactive mutant

Application Details

  • Application notes: See the Raf KI FF knockout mouse that these cells were derived from in related products.

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

Related Tools

Product Image

PEA1 Cell Line

#151678
Product Image

OVCAR-8/EGFP_LC3 - Mito DsRed

#161160
Product Image

ZR-75-1 [IX-23] cell line

#154550

References

  • •  Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
  • •  MEK kinase activity is not necessary for Raf-1 function.