CELL LINES
Contributor Information
- Name Catrin Pritchard
- Institute University of Leicester
Tool Details
- Tool name: C-Raf KI D486A Cell Line
- Tool type: Cell Lines
- Parental cell line: See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Model: Knock-In
- Description: The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling. See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
- Research area: Cancer; Cell signaling and signal transduction; Drug development
- Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4ÄÂ?°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
- For Research Use Only
Target Details
- Target: Raf1 D486A oncogenic mutant
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
- • CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
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