CELL LINES
Contributor Information
- Name Peter Parker
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: MEF PKCe KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer; Heart disease
- Model: Knock-Out
- Description: The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+. Matched isogenic cell line with PKCe re-expressed is also available. Cells have reduced migratory capacity compared to their PKCe-expressing counterparts. Cells were used to demonstrate a role for PKCe in controlling the traffic of beta1 integrins in motile cells
- Research area: Cancer; Adhesion; Cell Signaling & Signal Transduction; Cell Structure and Motility
- Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution.
- Cellosaurus ID: CVCL_AS81
- For Research Use Only
Target Details
- Target: PKC epsilon
Application Details
Handling
- Format: Frozen
- Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Cited by 24 subsequent publications including:
- • Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
- • Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
- • Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
- • FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.
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