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Contributor Information

  • Name Peter Parker
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MEF PKCe KO Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer; Heart disease
  • Model: Knock-Out
  • Description: The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+. Matched isogenic cell line with PKCe re-expressed is also available. Cells have reduced migratory capacity compared to their PKCe-expressing counterparts. Cells were used to demonstrate a role for PKCe in controlling the traffic of beta1 integrins in motile cells
  • Research area: Cancer; Adhesion; Cell Signaling & Signal Transduction; Cell Structure and Motility
  • Production details: MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution.
  • Cellosaurus ID: CVCL_AS81

  • For Research Use Only

Target Details

  • Target: PKC epsilon

Application Details


  • Format: Frozen
  • Growth medium: Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
  • Shipping conditions: Dry ice


  • Available on request


  •   Cited by 24 subsequent publications including:
  •   Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
  •   Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
  •   Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
  •   FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.