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Contributor Information

  • Name Simon Langdon
  • Institute Cancer Research UK Edinburgh Centre

Tool Details

  • Tool name: PEO1 Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Adenocarcinoma
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: The PEO1 Cell Line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO1 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO1 is tumourigenic in immunologically-deprived CBA mice. PEO1 exhibits good growth in semi-solid medium (agar). PEO1 is from the same patient as the PEO4 and PEO6 cell lines
  • Research area: Cancer ; Drug Discovery & Development
  • Cellosaurus ID: CVCL_2686

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum. Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 37 hours.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Cruz et al. 2017. Cancer Genomics Proteomics. 14(1):35-51. PMID: 28031236.
  •   Proteomics Analysis of Ovarian Cancer Cell Lines and Tissues Reveals Drug Resistance-associated Proteins.
  •   Coscia et al. 2016. Nat Commun. 7:12645. PMID: 27561551.
  •   Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status.
  •   Matassa et al. 2016. Cell Death Differ. :. PMID: 27206315.
  •   Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer.
  •   A comprehensive survey of the mutagenic impact of common cancer cytotoxics.
  •   Szikriszt et al. 2016. Genome Biol. 17(1):99. PMID: 27161042.
  •   The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer.
  •   A Systems Oncology Approach Identifies NT5E as a Key Metabolic Regulator in Tumor Cells and Modulator of Platinum Sensitivity.
  •   Huang et al. 2015. Oncotarget. :. PMID: 26683361.
  •   Nevedomskaya et al. 2015. J Proteome Res. :. PMID: 26629888.
  •   Patel et al. 2015. Cell Oncol (Dordr). :. PMID: 26266765.
  •   Metformin and epithelial ovarian cancer therapeutics.
  •   Hearn et al. 2015. Proc Natl Acad Sci U S A. :. PMID: 26162681.
  •   Potent organo-osmium compound shifts metabolism in epithelial ovarian cancer cells.
  •   Ren et al. 2015. J Steroid Biochem Mol Biol. 150:54-63. PMID: 25817828.
  •   Local estrogen metabolism in epithelial ovarian cancer suggests novel targets for therapy.
  •   Huntoon et al. 2013. Cancer Res. 73(12):3683-91. PMID: 23548269.
  •   ATR inhibition broadly sensitizes ovarian cancer cells to chemotherapy independent of BRCA status.
  •   Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.
  •   Oestrogen receptor expression and the effects of oestrogen and tamoxifen on the growth of human ovarian carcinoma cell lines.
  •   Langdon et al. 1988. Cancer Res. 48(21):6161-5. PMID: 3167862.
  •   Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863.
  •   Effect of sodium butyrate and other differentiation inducers on poorly differentiated human ovarian adenocarcinoma cell lines.
  •   Characterization and properties of nine human ovarian adenocarcinoma cell lines.