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Contributor Information

  • Name Simon Langdon
  • Institute Cancer Research UK Edinburgh Centre

Tool Details

  • Tool name: PEO4 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Adenocarcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Description: The PEO4 cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO4 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO4 was collected after clinical resistance developed to chemotherapy. PEO4 is tumourigenic in immunologically-deprived CBA mice. PEO4 exhibits good growth in semi-solid medium (agar). PEO4 is from the same patient as the PEO1 and PEO6 cell lines
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum. Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 37 hours.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Matassa et al. 2016. Cell Death Differ. :. PMID: 27206315.
  •   Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer.
  •   The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer.
  •   Huang et al. 2015. Oncotarget. :. PMID: 26683361.
  •   Shields et al. 2015. Mol Syst Biol. 11(12):842. PMID: 26655797.
  •   A genome-scale screen reveals context-dependent ovarian cancer sensitivity to miRNA overexpression.
  •   Patel et al. 2015. Cell Oncol (Dordr). :. PMID: 26266765.
  •   Metformin and epithelial ovarian cancer therapeutics.
  •   Hearn et al. 2015. Proc Natl Acad Sci U S A. :. PMID: 26162681.
  •   Potent organo-osmium compound shifts metabolism in epithelial ovarian cancer cells.
  •   Tuthill et al. 2015. Oncogene. 34(16):2138-44. PMID: 24909167.
  •   TRAIL-R2-specific antibodies and recombinant TRAIL can synergise to kill cancer cells.
  •   Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.
  •   Oestrogen receptor expression and the effects of oestrogen and tamoxifen on the growth of human ovarian carcinoma cell lines.
  •   Langdon et al. 1988. Cancer Res. 48(21):6161-5. PMID: 3167862.
  •   Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863.
  •   Effect of sodium butyrate and other differentiation inducers on poorly differentiated human ovarian adenocarcinoma cell lines.
  •   Characterization and properties of nine human ovarian adenocarcinoma cell lines.