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Contributor Information

  • Name Simon Langdon
  • Institute Cancer Research UK Edinburgh Centre

Tool Details

  • Tool name: PEO6 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Adenocarcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Description: The PEO6 cell line is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO6 was collected at advanced, terminal stage disease. PEO6 is tumourigenic in immunologically-deprived CBA mice. PEO6 exhibits good growth in semi-solid medium (agar). PEO6 is from the same patient as the PEO1 and PEO4 cell lines This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 77 hours. RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Matassa et al. 2016. Cell Death Differ. :. PMID: 27206315.
  •   Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer.
  •   Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.
  •   Oestrogen receptor expression and the effects of oestrogen and tamoxifen on the growth of human ovarian carcinoma cell lines.
  •   Degos et al. 1979. Gastroenterol Clin Biol. 3(10):735-9. PMID: 316786.
  •   [The natural history of gastrointestinal bleeding in patients with alcoholic cirrhosis. Definition of a subgroup of patients with high risk of bleeding recurrence (author's transl)]